Investigation of protein expression of <i>Saccharomyces cerevisiae</i> cells in quiescent and proliferating state before and after toxic stress

The focus of the present study is to determine proteins responsible for oxidative and toxic stress response in proliferating stationary phase (G0) cultures. Therefore, yeast Saccharomyces cerevisiae was treated with drug compounds (H2O2, menadione, zeocin, ibuprofen) both phases. These substances were chosen redox status yeast. S. appeared employ different strategies ensure their antioxidant defence metabolism. Analysis, including sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) coupled mass spectrometry, used search. identified by SDS-PAGE, matrix-assisted laser desorption/ionization time-of-flight/time-of-flight (MALDI-TOF/TOF) spectrometry analysis, Mascot database-fingerprint. final step determination protein profiles (M) (G0). Seven bands determined corresponding proposed: cytochrome c peroxidase, glutathione S-transferase omega-like, NAPDH-dependent diflavin reductase, DNA replication fork-blocking protein, putative aryl alcohol dehydrogenase, AP-1-like transcription factor YAP5, GTP-binding protein. All coincide literature database. A typical example such an adaptation mechanism against damage synthesis several thioredoxin peroxidases cell. deeper investigation conserved mechanisms entry into, survival, exit from quiescence higher eukaryotes will help development new anticancer therapies, process ageing neurodegenerative diseases.